rabbit polyclonal anti ror2  (OriGene)

Journal: bioRxiv

Article Title: The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation

doi: 10.1101/2021.03.02.433549

Figure Lengend Snippet: Cells expressing Ror2 in cardiac tissue was visualized in Ror2-LacZ mice 28 days after A) sham surgery or B) TAC surgery. C) Protein localization was visualized in cardiac tissue 7 days after TAC by IHC for Ror2. D) Ror1 and Ror2 protein expression was measured by western blot at various days after TAC surgery (β-actin as a loading control). E) Endothelial cells, leukocytes, and fibroblasts were isolated 7 days after Sham or TAC surgery, and RNA expression of relevant genes was quantified.

Article Snippet: Cardiac tissue sections were either stained for 5-bromo-4-chloro-3-indolyl-b-galactosidase (Xgal; Roche Cat #XGAL-RO) or immunostained with rabbit anti-mouse polyclonal Ror2 (provided by Dr. Yasuhiro Minami, Kobe University) with DAB visualization (Vector Laboratories, Cat #SK-4100), and counterstained with hematoxylin and eosin (Sigma Aldrich, Cat #HHS128-4L and #HT110180-2.5L, respectively).

Techniques: Expressing, Western Blot, Isolation, RNA Expression

Journal: Annals of Translational Medicine

Article Title: Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription

doi: 10.21037/atm-22-4583

Figure Lengend Snippet: ROR2 was upregulated in gastric cancer and associated with poor prognosis. (A) Expression profiling of ROR2 mRNA in gastric tumor specimens compared with matched normal gastric tissues in a cohort of gastric cancer specimens (n=124). Waterfall plot shows the log values of gastric tumor specimens divided by matched normal gastric tissues. Scatter dot plots show the relative mRNA expression of ROR2 in matched gastric cancer tissues (n=124). (B) Representative photographs at 200× magnification of IHC staining in 81 gastric cancer samples. (C) Kaplan-Meier curves for OS and DFS of 81 gastric cancer patients with high or low ROR2 expression. *P<0.05. IHC, immunohistochemistry; OS, overall survival; DFS, disease-free survival.

Article Snippet: Rabbit anti-human ROR2 polyclonal antibody was purchased from Abgent Company (San Diego, USA).

Techniques: Expressing, Immunohistochemistry

Journal: Annals of Translational Medicine

Article Title: Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription

doi: 10.21037/atm-22-4583

Figure Lengend Snippet: Clinical characteristics of the patients with gastric cancer

Article Snippet: Rabbit anti-human ROR2 polyclonal antibody was purchased from Abgent Company (San Diego, USA).

Techniques: Expressing

Journal: Annals of Translational Medicine

Article Title: Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription

doi: 10.21037/atm-22-4583

Figure Lengend Snippet: Univariate analyses of predictors of disease-free survival and overall survival in patients with gastric cancer

Article Snippet: Rabbit anti-human ROR2 polyclonal antibody was purchased from Abgent Company (San Diego, USA).

Techniques:

Journal: Annals of Translational Medicine

Article Title: Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription

doi: 10.21037/atm-22-4583

Figure Lengend Snippet: The ROR2 expression in gastric cancer cell lines was detected. (A) ROR2 mRNA and protein expression in gastric cancer cell lines and immortalized normal gastric mucosal epithelial cell line via qRT-PCR and western blot. (B) ROR2 expression was determined by qRT-PCR and western blot after ROR2-overexpression vector and shROR2 vector were transfected into gastric cell lines. qRT-PCR, quantitative real-time polymerase chain reaction; ROR2 WT and Con, ROR2-overexpressing (WT) and the empty vector plasmids (Con); shROR2 and NC, short hairpin (shRNA) targeting ROR2 (shROR2) and the empty vector plasmids (NC).

Article Snippet: Rabbit anti-human ROR2 polyclonal antibody was purchased from Abgent Company (San Diego, USA).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Over Expression, Plasmid Preparation, Transfection, Real-time Polymerase Chain Reaction, shRNA

Journal: Annals of Translational Medicine

Article Title: Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription

doi: 10.21037/atm-22-4583

Figure Lengend Snippet: ROR2 promoted gastric cancer cell migration, invasion, and metastasis. (A,C) SNU601 cells stably expressing ROR2 WT and Con as well as SGC7901 cells stably expressing shROR2 and NC were subjected to transwell migration (A) and invasion assays (C). Representative images at 200× magnification and quantitative analysis of transwell assays after crystal violet staining are presented. Columns are the mean of 3 independent experiments, and bars = SD. (B) Wound-healing assay. The red lines indicate the initial scratch wound location, while the yellow blocks show unhealed areas left by migratory cells. Images were captured at the indicated times after wounding. Representative images and quantitative analysis of relative wound density are presented. (D) Three-dimensional culture assay. Representative images at 100× magnification and 200× magnification show the spheres formed by SNU601-ROR2 WT and Con. (E) SNU601 cells stably expressing ROR2 WT and Con were respectively injected into BALB/c nude mice through the tail vein. Representative images at 100× magnification (upper) and 200× magnification (lower) of HE staining of lung sections are shown (left). Quantitative analysis of the number of metastatic tumors in the lungs of nude mice (upper right) and lung weight (lower right). Columns are the mean of 3 independent experiments, and bars = SD. *, P<0.05; **, P<0.01; ***, P<0.001. SD, standard deviation; ROR2 WT and Con, ROR2-overexpressing (WT) and the empty vector plasmids (Con); shROR2 and NC, short hairpin (shRNA) targeting ROR2 (shROR2) and the empty vector plasmids (NC); HE, hematoxylin and eosin.

Article Snippet: Rabbit anti-human ROR2 polyclonal antibody was purchased from Abgent Company (San Diego, USA).

Techniques: Migration, Stable Transfection, Expressing, Staining, Wound Healing Assay, Injection, Standard Deviation, Plasmid Preparation, shRNA

Journal: Annals of Translational Medicine

Article Title: Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription

doi: 10.21037/atm-22-4583

Figure Lengend Snippet: Screening for ROR2-modulating genes. (A) Signaling explorer antibody microarray of SNU216-ROR2 WT and SNU216-Con. The heat map is presented. (B) Representative qRT-PCR images of 8 genes closely related to tumor proliferation and migration were examined at the mRNA level according to the signaling explorer antibody microarray. qRT-PCR was conducted using AGS-ROR2 WT, GTL16-ROR2 WT, SNU216-ROR2 WT, SNU601-ROR2 WT, SGC7901-shROR2, MGC803-shROR2, and their respective control cells. Relative mRNA expressions are presented in groups. qRT-PCR, quantitative real-time polymerase chain reaction; ROR2 WT and Con, ROR2-overexpressing (WT) and the empty vector plasmids (Con); shROR2 and NC, short hairpin (shRNA) targeting ROR2 (shROR2) and the empty vector plasmids (NC).

Article Snippet: Rabbit anti-human ROR2 polyclonal antibody was purchased from Abgent Company (San Diego, USA).

Techniques: Microarray, Quantitative RT-PCR, Migration, Real-time Polymerase Chain Reaction, Plasmid Preparation, shRNA

Journal: Annals of Translational Medicine

Article Title: Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription

doi: 10.21037/atm-22-4583

Figure Lengend Snippet: MMP3 as a key downstream factor of ROR2. (A) ELISA was conducted to detect the MMP3 protein expression in GTL16 and SNU601 cells stably expressing ROR2 WT and Con as well as SGC7901 and MGC803 cells stably expressing shROR2 and NC. (B) Detection of MMP3 protein expression by western blot in SNU601 cells stably expressing ROR2 WT and Con, as well as in SGC7901 cells stably expressing shROR2 and NC. (C) Spearman correlation analysis of the mRNA expression of ROR2 and MMP3 in 32 cases of gastric cancer. **, P<0.01; ***P<0.001. ELISA, enzyme-linked immunosorbent assay; ROR2 WT and Con, ROR2-overexpressing (WT) and the empty vector plasmids (Con); shROR2 and NC, short hairpin (shRNA) targeting ROR2 (shROR2) and the empty vector plasmids (NC).

Article Snippet: Rabbit anti-human ROR2 polyclonal antibody was purchased from Abgent Company (San Diego, USA).

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Stable Transfection, Western Blot, Plasmid Preparation, shRNA

Journal: Annals of Translational Medicine

Article Title: Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription

doi: 10.21037/atm-22-4583

Figure Lengend Snippet: ROR2 activated the non-canonical Wnt pathway but not the canonical Wnt pathway. (A,B) Western blot of canonical Wnt pathway proteins and non-canonical Wnt pathway proteins in AGS, SNU601 (A), SGC7901, and MGC803 (B) cells stably expressing ectopic ROR2 and the control vectors. (C) Western blot of JNK1/2 and p-JNK1/2 in nucleus proteins and cytoplasm proteins, respectively, from AGS-ROR2 WT and AGS-Con cells. ROR2 WT and Con, ROR2-overexpressing (WT) and the empty vector plasmids (Con); shROR2 and NC, short hairpin (shRNA) targeting ROR2 (shROR2) and the empty vector plasmids (NC).

Article Snippet: Rabbit anti-human ROR2 polyclonal antibody was purchased from Abgent Company (San Diego, USA).

Techniques: Western Blot, Stable Transfection, Expressing, Plasmid Preparation, shRNA

Journal: Annals of Translational Medicine

Article Title: Role of ROR2 in promoting gastric cancer metastasis by enhancing c-JUN-mediated MMP3 transcription

doi: 10.21037/atm-22-4583

Figure Lengend Snippet: c-JUN transcriptionally activates MMP3 . (A) Relative MMP3 mRNA expression in HEK293T cells and in those transfected with c-JUN overexpression plasmid. (B) Dual luciferase activity in HEK293T cells and AGS cells transfected with pGL3-MMP3 promoter region and c-JUN-overexpression or control plasmid. Columns are the mean of 3 independent experiments, and bars = SD. (C) Schematic diagram of the full length and 3 truncated fragments of the MMP3 promoter. (D) Dual luciferase activity of c-JUN and different fragments of MMP3 promoter. Columns are the mean of 3 independent experiments, and bars = SD. (E) ChIP qRT-PCR assay. Relative enrichment of 3 different regions of the MMP3 promoter precipitated with c-JUN in AGS-ROR2 WT and AGS-Con cells. *, P<0.05; **, P<0.01. SD, standard deviation; ChIP, chromatin immunoprecipitation; qRT-PCR, quantitative real-time polymerase chain reaction; ROR2 WT and Con, ROR2-overexpressing (WT) and the empty vector plasmids (Con).

Article Snippet: Rabbit anti-human ROR2 polyclonal antibody was purchased from Abgent Company (San Diego, USA).

Techniques: Expressing, Transfection, Over Expression, Plasmid Preparation, Luciferase, Activity Assay, Quantitative RT-PCR, Standard Deviation, Chromatin Immunoprecipitation, Real-time Polymerase Chain Reaction

Journal: bioRxiv

Article Title: The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation

doi: 10.1101/2021.03.02.433549

Figure Lengend Snippet: Cells expressing Ror2 in cardiac tissue was visualized in Ror2-LacZ mice 28 days after A) sham surgery or B) TAC surgery. C) Protein localization was visualized in cardiac tissue 7 days after TAC by IHC for Ror2. D) Ror1 and Ror2 protein expression was measured by western blot at various days after TAC surgery (β-actin as a loading control). E) Endothelial cells, leukocytes, and fibroblasts were isolated 7 days after Sham or TAC surgery, and RNA expression of relevant genes was quantified.

Article Snippet: Protein lysates from TAC heart samples were isolated from cardiac tissue by 1% Triton-X supplemented with protease inhibitors and probed for rabbit anti-mouse polyclonal Ror1 (provided by Dr. Michael Greenberg, ) rabbit anti-mouse monoclonal Ror2 (Cell Signaling Technology Cat #88639), or β-actin (Cell Signaling Technology Cat #4970) by western blot.

Techniques: Expressing, Western Blot, Control, Isolation, RNA Expression

Journal: bioRxiv

Article Title: The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation

doi: 10.1101/2021.03.02.433549

Figure Lengend Snippet: Murine cardiac fibroblasts were isolated from healthy cardiac tissue of C57Bl6 mice. A) RNA expression of genes associated with fibroblast identity, fibroblast activation, Ror1/2 receptors, and planar cell polarity signaling was quantified over time. Cardiac myofibroblasts at passage 2 were stained for SMØ-actin fibers after B) control and C) TGF-β1 treatment. D) RNA expression of genes associated with myofibroblast differentiation, inflammation, and Ror1/2 receptors was quantified in control and TGF-β1 treated fibroblasts.

Article Snippet: Protein lysates from TAC heart samples were isolated from cardiac tissue by 1% Triton-X supplemented with protease inhibitors and probed for rabbit anti-mouse polyclonal Ror1 (provided by Dr. Michael Greenberg, ) rabbit anti-mouse monoclonal Ror2 (Cell Signaling Technology Cat #88639), or β-actin (Cell Signaling Technology Cat #4970) by western blot.

Techniques: Isolation, RNA Expression, Activation Assay, Staining, Control

Journal: bioRxiv

Article Title: The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation

doi: 10.1101/2021.03.02.433549

Figure Lengend Snippet: Primary cardiac fibroblasts were isolated from healthy control and transgenic Ror1/2 double-knockout mice transcriptional phenotype was assessed by bulk RNA sequencing. A) RNA transcript reads of specific genes related to planar cell polarity, inflammatory cytokines, proliferation and cell division, ERK1/2 signaling, matrix remodeling, natriuretic signaling, and myofibroblast differentiation were quantified as normalized Z-score (4 samples for each genotype). B) Gene ontology analysis of differential gene expression between control and Ror1/2-KO cardiac fibroblasts showed the top 10 up-regulated and down-regulated terms by q-value, with terms grouped by color: cell activation in orange, inflammation in red, proliferation in green, and microtubule regulation in black. C) Significance versus fold change of each gene between control and Ror1/2-KO samples was visualized by volcano plot, with genes in each gene ontology term highlighted in corresponding colors (all other genes in grey).

Article Snippet: Protein lysates from TAC heart samples were isolated from cardiac tissue by 1% Triton-X supplemented with protease inhibitors and probed for rabbit anti-mouse polyclonal Ror1 (provided by Dr. Michael Greenberg, ) rabbit anti-mouse monoclonal Ror2 (Cell Signaling Technology Cat #88639), or β-actin (Cell Signaling Technology Cat #4970) by western blot.

Techniques: Isolation, Control, Transgenic Assay, Double Knockout, RNA Sequencing, Gene Expression, Activation Assay

Journal: bioRxiv

Article Title: The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation

doi: 10.1101/2021.03.02.433549

Figure Lengend Snippet: Myofibroblast differentiation was induced by treatment with 10ng/mL TGF-β1 for 4 days. A) RNA expression of myofibroblast-related (Acta2 and Postn) and inflammation-related (IL-6) genes was quantified in control and Ror1/2-KO fibroblasts. B) SMΓ]-actin filaments were visualized by immunofluorescent staining of sub-confluent cells in control and Ror1/2-KO fibroblasts, and C) alignment of SMΓ1-actin filaments was quantified and normalized per cell.

Article Snippet: Protein lysates from TAC heart samples were isolated from cardiac tissue by 1% Triton-X supplemented with protease inhibitors and probed for rabbit anti-mouse polyclonal Ror1 (provided by Dr. Michael Greenberg, ) rabbit anti-mouse monoclonal Ror2 (Cell Signaling Technology Cat #88639), or β-actin (Cell Signaling Technology Cat #4970) by western blot.

Techniques: RNA Expression, Control, Staining

Journal: bioRxiv

Article Title: The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation

doi: 10.1101/2021.03.02.433549

Figure Lengend Snippet: A) Transgenic Ror1/2 double knockout and control mice were subjected to TAC surgery, and cardiac output was imaged by echocardiography. B) Cardiac output factors were quantified: fractional shortening, left ventricular posterior wall thickness at end diastole, left ventricular diameter at end systole, and left ventricular diameter at end diastole. C) Survival of Ror1/2 double knockout mice and control mice after TAC surgery was recorded each day.

Article Snippet: Protein lysates from TAC heart samples were isolated from cardiac tissue by 1% Triton-X supplemented with protease inhibitors and probed for rabbit anti-mouse polyclonal Ror1 (provided by Dr. Michael Greenberg, ) rabbit anti-mouse monoclonal Ror2 (Cell Signaling Technology Cat #88639), or β-actin (Cell Signaling Technology Cat #4970) by western blot.

Techniques: Transgenic Assay, Double Knockout, Control

Journal: bioRxiv

Article Title: The Cell Surface Receptors Ror1/2 Control Cardiac Myofibroblast Differentiation

doi: 10.1101/2021.03.02.433549

Figure Lengend Snippet: Inflammatory profile of control and transgenic Ror1/2 double knockout mice was assessed after TAC or sham surgery. A) H&E staining of cardiac tissues 3-days post-TAC were imaged. B) Cells were isolated from cardiac tissue and relative quantity of leukocyte populations were determined by flow cytometry, C) quantified by absolute number. D) Gene expression of pro-inflammatory cytokines in cardiac lysate was measured. E) Vascular permeability at 1-day post-TAC was assessed by Evans Blue dye injection, with vascular leakage visualized by blue dye in the cardiac tissue.

Article Snippet: Protein lysates from TAC heart samples were isolated from cardiac tissue by 1% Triton-X supplemented with protease inhibitors and probed for rabbit anti-mouse polyclonal Ror1 (provided by Dr. Michael Greenberg, ) rabbit anti-mouse monoclonal Ror2 (Cell Signaling Technology Cat #88639), or β-actin (Cell Signaling Technology Cat #4970) by western blot.

Techniques: Control, Transgenic Assay, Double Knockout, Staining, Isolation, Flow Cytometry, Gene Expression, Permeability, Injection

Journal: Oncology Letters

Article Title: Higher expression level of tyrosine kinase-like orphan receptor 2 and Wnt member 5a in papillary thyroid carcinoma is associated with poor prognosis

doi: 10.3892/ol.2017.6989

Figure Lengend Snippet: The expression level of tyrosine kinase-like orphan receptor 2 in (A) normal thyroid tissues and (B) papillary thyroid carcinoma tissues by immunohistochemistry (magnification, ×200).

Article Snippet: The rabbit anti-human ROR2 polyclonal antibody (cat. no. SC-98486; 1:1,000) and the rabbit anti-human β-actin polyclonal antibodies (cat. no. SC-130656; 1:1,000) were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

Techniques: Expressing, Immunohistochemistry

Journal: Oncology Letters

Article Title: Higher expression level of tyrosine kinase-like orphan receptor 2 and Wnt member 5a in papillary thyroid carcinoma is associated with poor prognosis

doi: 10.3892/ol.2017.6989

Figure Lengend Snippet: Analysis of ROR2 expression level in PTC and normal tissues.

Article Snippet: The rabbit anti-human ROR2 polyclonal antibody (cat. no. SC-98486; 1:1,000) and the rabbit anti-human β-actin polyclonal antibodies (cat. no. SC-130656; 1:1,000) were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Higher expression level of tyrosine kinase-like orphan receptor 2 and Wnt member 5a in papillary thyroid carcinoma is associated with poor prognosis

doi: 10.3892/ol.2017.6989

Figure Lengend Snippet: The expression level of ROR2 in PTC tissues and the corresponding normal tissues. A relatively high ROR2 protein expression level was revealed in PTC tissues compared with in normal thyroid tissues (*P<0.05). ROR2, tyrosine kinase-like orphan receptor 2; PTC, papillary thyroid carcinoma.

Article Snippet: The rabbit anti-human ROR2 polyclonal antibody (cat. no. SC-98486; 1:1,000) and the rabbit anti-human β-actin polyclonal antibodies (cat. no. SC-130656; 1:1,000) were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Higher expression level of tyrosine kinase-like orphan receptor 2 and Wnt member 5a in papillary thyroid carcinoma is associated with poor prognosis

doi: 10.3892/ol.2017.6989

Figure Lengend Snippet: The expression levels of ROR2 mRNA in PTC tissues and its counterpart normal tissues were detected by reverse transcription-quantitative polymerase chain reaction (*P<0.05). ROR2, tyrosine kinase-like orphan receptor 2; PTC, papillary thyroid carcinoma.

Article Snippet: The rabbit anti-human ROR2 polyclonal antibody (cat. no. SC-98486; 1:1,000) and the rabbit anti-human β-actin polyclonal antibodies (cat. no. SC-130656; 1:1,000) were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: Higher expression level of tyrosine kinase-like orphan receptor 2 and Wnt member 5a in papillary thyroid carcinoma is associated with poor prognosis

doi: 10.3892/ol.2017.6989

Figure Lengend Snippet: Association between ROR2 expression level and clinicopathological characteristics in patients with PTC.

Article Snippet: The rabbit anti-human ROR2 polyclonal antibody (cat. no. SC-98486; 1:1,000) and the rabbit anti-human β-actin polyclonal antibodies (cat. no. SC-130656; 1:1,000) were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Higher expression level of tyrosine kinase-like orphan receptor 2 and Wnt member 5a in papillary thyroid carcinoma is associated with poor prognosis

doi: 10.3892/ol.2017.6989

Figure Lengend Snippet: Association between ROR2 and Wnt5a expression levels in PTC.

Article Snippet: The rabbit anti-human ROR2 polyclonal antibody (cat. no. SC-98486; 1:1,000) and the rabbit anti-human β-actin polyclonal antibodies (cat. no. SC-130656; 1:1,000) were purchased from Santa Cruz Biotechnology Inc. (Dallas, TX, USA).

Techniques: Expressing

Journal: Scientific Reports

Article Title: High ROR2 expression in tumor cells and stroma is correlated with poor prognosis in pancreatic ductal adenocarcinoma

doi: 10.1038/srep12991

Figure Lengend Snippet: Two PDAC cell lines (BXPC3, PNAC-1) have higher ROR2 expression than the benign pancreatic ductal cell line (HPDE6C7). The mean expression level of ROR2 mRNA in cancerous tissue was higher on average in the tumor tissues than in corresponding non-malignant tissues.

Article Snippet: TMA slides were separately stained using polyclonal rabbit anti-ROR2 antibody (LifeSpan BioSciences Inc., Seattle, WA, USA).

Techniques: Expressing

Journal: Scientific Reports

Article Title: High ROR2 expression in tumor cells and stroma is correlated with poor prognosis in pancreatic ductal adenocarcinoma

doi: 10.1038/srep12991

Figure Lengend Snippet: ( a1 , a2 ) Positive tumor cytoplasmic (red arrow) and negative stromal (blue arrow) immunohistochemical staining of ROR2 in PDAC samples. ( b1 , b2 ) Strong tumor (red arrow) and stromal (green arrow) immunohistochemical staining of ROR2 in PDAC samples. ( c1 , c2 ) Negative IHC of ROR2 in tumor cells (black arrow) with strong positive stromal (green arrow) staining in PDAC tissue samples. ( d1 , d2 ) There was negative immunohistochemical staining of ROR2 in cancer cells (black arrow) and stromal cells (blue arrow). ( e1 , e2 ) Negative staining of ROR2 in epithelial cells (black arrow) in benign pancreatic disease tissues. ( f1 , f2 ) Negative immunohistochemical staining of ROR2 in benign pancreatic ductal cells (black arrow). Original magnification ×40 (bar = 500 μm) in ( a1 , b1 , c1 , d1 , e1 and f1 ); ×400 (bar = 50 μm) in ( a2 , b2 , c2 , d2 , e2 and f2 ).

Article Snippet: TMA slides were separately stained using polyclonal rabbit anti-ROR2 antibody (LifeSpan BioSciences Inc., Seattle, WA, USA).

Techniques: Immunohistochemical staining, Staining, Negative Staining

Journal: Scientific Reports

Article Title: High ROR2 expression in tumor cells and stroma is correlated with poor prognosis in pancreatic ductal adenocarcinoma

doi: 10.1038/srep12991

Figure Lengend Snippet: Association of ROR2 expression with clinical attributes of pancreatic ductal adenocarcinoma.

Article Snippet: TMA slides were separately stained using polyclonal rabbit anti-ROR2 antibody (LifeSpan BioSciences Inc., Seattle, WA, USA).

Techniques: Expressing, Staining

Journal: Scientific Reports

Article Title: High ROR2 expression in tumor cells and stroma is correlated with poor prognosis in pancreatic ductal adenocarcinoma

doi: 10.1038/srep12991

Figure Lengend Snippet: Univariate and multivariate analysis of prognostic factors for 5-year survival in pancreatic cancer.

Article Snippet: TMA slides were separately stained using polyclonal rabbit anti-ROR2 antibody (LifeSpan BioSciences Inc., Seattle, WA, USA).

Techniques: Expressing, Staining

Journal: Scientific Reports

Article Title: High ROR2 expression in tumor cells and stroma is correlated with poor prognosis in pancreatic ductal adenocarcinoma

doi: 10.1038/srep12991

Figure Lengend Snippet: ( a ) Overall survival rate in patients with high cytoplasmic ROR2 expression (green line) was significantly lower than in patients with low or no cytoplasmic ROR2 expression (blue line). ( b ) Overall survival rate in patients with high stromal ROR2 expression (green line) was significantly lower than in patients with low or no stromal ROR2 expression (blue line).

Article Snippet: TMA slides were separately stained using polyclonal rabbit anti-ROR2 antibody (LifeSpan BioSciences Inc., Seattle, WA, USA).

Techniques: Expressing

Journal: Laboratory investigation; a journal of technical methods and pathology

Article Title: Extracellular calcium-sensing receptor/PTH knockout mice colons have increased Wnt/β-catenin signaling, reduced non-canonical Wnt signaling, and increased susceptibility to azoxymethane-induced aberrant crypt foci.

doi: 10.1038/labinvest.2013.51

Figure Lengend Snippet: Figure 3 Western analyses of Wnt5a, Ror2, and TNFR1 in C /P and C þ /P þ colonic epithelial cells. (a) Wnt5a reduced four-fold in C /P compared with C þ /P þ colonic epithelia. (b) Ror2 expression reduced 11-fold in C /P compared with C þ /P þ colonic epithelia. (c) TNFR1 increased three-fold in C /P compared with epithelia of C þ /P þ mice. All changes significant. (Po0.05, n ¼ 6).

Article Snippet: Anti-Ror2 rabbit polyclonal antibody (1:1000) and anti-TNFR1 (1:1000) were from Cell Signaling Technology (Boston, MA, USA).

Techniques: Western Blot, Expressing